--- title: Absolute Precision in Protein Analysis | Digital PCR Solutions | PICO description: PICO offers precise protein quantification and analysis, streamlining workflows for detecting interactions and characterizing extracellular vesicles with minimal sample requirements. --- [Skip to content](https://www.pico-bioscience.eu#main-content) [![PICO BioScience logo](https://www.pico-bioscience.eu/hs-fs/hubfs/Logos/PICO%20and%20actome/PICO%20BioScience%20(no%20background).png?width=1280&height=722&name=PICO%20BioScience%20(no%20background).png)Homepage](https://www.pico-bioscience.eu) - Solutions - [Single EV Analysis](https://www.pico-bioscience.eu/ev-analysis?hsLang=eu) - [Protein Detection](https://www.pico-bioscience.eu/protein-detection?hsLang=eu) - [Antibody Labeling](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) - The PICO Workflow - [PICO Technology Overview](https://www.pico-bioscience.eu/technology-pico-bioscience?hsLang=eu) - [PIQuant Software](https://www.pico-bioscience.eu/piquant?hsLang=eu) - [Instrument Compatibility](https://www.pico-bioscience.eu/instrument-compatibility?hsLang=eu) - [Resources](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Application Notes](https://www.pico-bioscience.eu/application-notes?hsLang=eu) - [Technical Support](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Manuals](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Company](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) - [Our Mission](https://www.pico-bioscience.eu/our-mission?hsLang=eu) - [Careers](https://www.linkedin.com/company/pico-bioscience/) - [Partnerships](https://www.pico-bioscience.eu/collaborative-research?hsLang=eu) - [Contact](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) - Solutions - [Single EV Analysis](https://www.pico-bioscience.eu/ev-analysis?hsLang=eu) - [Protein Detection](https://www.pico-bioscience.eu/protein-detection?hsLang=eu) - [Antibody Labeling](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) - The PICO Workflow - [PICO Technology Overview](https://www.pico-bioscience.eu/technology-pico-bioscience?hsLang=eu) - [PIQuant Software](https://www.pico-bioscience.eu/piquant?hsLang=eu) - [Instrument Compatibility](https://www.pico-bioscience.eu/instrument-compatibility?hsLang=eu) - [Resources](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Application Notes](https://www.pico-bioscience.eu/application-notes?hsLang=eu) - [Technical Support](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Manuals](https://www.pico-bioscience.eu/resources?hsLang=eu) - [Company](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) - [Our Mission](https://www.pico-bioscience.eu/our-mission?hsLang=eu) - [Careers](https://www.linkedin.com/company/pico-bioscience/) - [Partnerships](https://www.pico-bioscience.eu/collaborative-research?hsLang=eu) - [Contact](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) [Shop](https://pico-bioscience.myshopify.com)[PIQuant](https://pico-bioscience-piquant.share.connect.posit.cloud/) # **Absolute Precision. From Single Proteins to Complex Interactions.** PICO redefines precision by translating protein status into digital PCR signals. Whether you are quantifying **low-abundance proteins**, mapping **Protein-Protein Interactions (PPIs)**, or characterizing **Extracellular Vesicles (EVs)**, PICO delivers absolute data from as little as **1 µl of sample**. - **Beyond Quantification:** Detect and quantify molecular complexes and interactions with single-molecule resolution. - **Multiplexed EVs:** Analyze up to four markers per vesicle, independent of size. - **Streamlined Workflow:** Skip the washing steps and the noise. Get **publication-ready data** over night. [Contact us](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu)[Book a Call](https://cta-eu1.hubspot.com/web-interactives/public/v1/track/click?encryptedPayload=AVxigLI4ma1uRyCddXDPXibAagMjLLXGftk4Kzod4UNEWfz4JCyEmk%2B8QpD122gjZz%2FArh7zqVZSfNo419y5IqDR63MHsv79asJQ7TTgOr7DzuA4w%2FTZ46an5BXMTFG3OGPvo2kork7ca1v2x5YUVDtn6CEAKGbxsI%2FFUMioh2lT5ss%2F6103F7Eoz%2F6r5TiNyRcNZf0%3D&portalId=25306495) [PIQuant Software](https://pico-bioscience.shinyapps.io/piquant)[Manuals & Support](https://www.pico-bioscience.eu/resources?hsLang=eu) ### The PICO Workflow --- [![Step1 - Labeling](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-1-Step1-%20Labeling.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-1-Step1-%20Labeling.gif "Step1 - Labeling")](https://landing.actome.de/antibody-labeling) #### **PICO Labeling Service: We Do the Work, You Get the Data** Don't waste time on manual conjugation. Name your targets, and our scientists will handle the entire process from procurement to strict quality control. - **Hands-Off Convenience:** Simply ship your antibodies to our lab, or let us order them directly from your preferred supplier. - **Tailored to Your Assay:** Choose the exact labels your specific multiplex assay requires. - **100% Site-Directed Precision:** We guarantee flawless, reproducible coupling targeting non-variable regions—leaving your antigen-binding sites completely untouched. - **Exact Stoichiometry:** Our standardized process ensures absolute molecular consistency, loading exactly 2 labels per heavy chain. - **Built-In Quality Control:** Every batch undergoes rigorous testing to verify the final concentration and absolute labeling efficiency. **Requirement:** This premium service is optimized for **IgG antibodies**. --- #### **The PICO Binding Reaction** No complex washing steps, no surface immobilization. Just mix your purified sample with the labeled antibodies and let the molecular proximity do the rest. - **Solution-Based Binding:** The entire reaction happens completely in solution. Your targets maintain their native conformation, ensuring realistic, unhindered biological interactions. - **No Surface Loss:** Because there are no washing steps or solid-phase binding, you completely eliminate the background noise and sample loss typical of traditional assays. - **Intact Membrane Integrity:** For EV analysis, the entire binding reaction is completely detergent-free. Your vesicles remain 100% intact from the initial mix to the final readout. ![Step2 - Binding](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-2-Step2-%20Binding%201.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-2-Step2-%20Binding%201.gif "Step2 - Binding") --- ![Step3 - Amplification](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-3-Step3-%20Amplification.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-3-Step3-%20Amplification.gif "Step3 - Amplification") #### **Digital PCR Partitioning & Multiplex Amplification** This is where the biochemistry turns into digital data. The sample mixture is loaded directly into the digital PCR system, whether it utilizes microfluidic nanoplates, chips, or droplet generation. - **Micro-Partitioning:** The sample is instantly distributed across thousands of isolated nanoliter cavities or oil droplets. Individual targets and their bound DNA-labeled antibodies are locked into separate, independent micro-reactions. - **Proximity-Driven Amplification:** Once partitioned, the standard dPCR master mix amplifies the DNA barcodes. A distinct fluorescent signal is generated *only* if the specific target markers are present together in that individual partition. - **Multiplexing Opportunities:** By using the multiple fluorescent dye channels on your dPCR hardware, you can simultaneously detect different target combinations, subpopulation markers, or distinct protein complexes in a single run. - **Direct Molecular Counting:** Because dPCR operates on a binary (positive/negative) readout per partition, the system counts the absolute number of fluorescent events. No reference standards or standard curves required. --- ### **Automated Data Analysis with PIQuant® Software** Once your digital PCR run is complete, the raw data is seamlessly converted into clear, actionable results without manual calculation or complex spreadsheets. - **Effortless Upload:** Simply upload the raw data files directly from your dPCR instrument into the intuitive PIQuant® software interface. - **Automated Partition Counting:** PIQuant® instantly scans and analyzes each cavity, compartment, or droplet, identifying the exact ratio of positive-to-negative fluorescent signals. - **Patented Quantification Algorithm:** The software utilizes a unique, patented algorithm to filter and calculate the absolute molecular concentration of your target complexes. - **Zero Calibration Needed:** Because the algorithm provides direct, absolute quantification, you get precise molecular counts without ever needing to run a standard reference curve. ![Step4 - Analysis](https://www.pico-bioscience.eu/hs-fs/hubfs/Schemes_Comparison/PICO-Workflow/Workflow%20f%C3%BCr%20Website%20und%20Presentation-4-Step4-%20Analysis.gif?width=500&height=500&name=Workflow%20f%C3%BCr%20Website%20und%20Presentation-4-Step4-%20Analysis.gif "Step4 - Analysis") ## How PICO makes it count! Video player - PICO BioScience - Click to play video ## Choose your target! - [![Unbenannt](https://www.pico-bioscience.eu/hs-fs/hubfs/Unbenannt.png?width=1080&height=1080&name=Unbenannt.png) **EV Quantification and Characterization**](https://www.pico-bioscience.eu/ev-analysis?hsLang=eu) - [![PICO\_single\_protein](https://www.pico-bioscience.eu/hs-fs/hubfs/PICO_single_protein.png?width=1080&height=1080&name=PICO_single_protein.png) **Protein Detection**](https://www.pico-bioscience.eu/protein-detection?hsLang=eu) - [![PICO\_Labeling\_Service](https://www.pico-bioscience.eu/hs-fs/hubfs/PICO_Labeling_Service.png?width=1080&height=1080&name=PICO_Labeling_Service.png) **Antibody Labeling Service**](https://www.pico-bioscience.eu/antibody-labeling?hsLang=eu) ## Key Advantages ![pipette](https://www.pico-bioscience.eu/hs-fs/hubfs/pipette.jpeg?width=803&height=536&name=pipette.jpeg "pipette") ### Only 1 µl of sample required ### High reproducibility without external reference standards ### Single EV characterization with up to four markers per vesicle ### Easy handling with minimal hands-on time ### Size-independent EV detection ![PICO Amplification Core Kit image](https://cdn.shopify.com/s/files/1/0042/1026/2051/files/Products-14-AMC_Kit.png?v=1781705393) ## PICO Amplification Core Kit 1.500,00 € The **PICO Amplification Core Kit** enables the detection and absolute quantification of Extracellular vesicles, proteins, protein interactions, and post-translational modifications. The workflow includes the lysis of the sample, followed by the incubation of lysates with the labeled antibodies. After the incubation the sample is highly diluted and loaded into a dPCR plate. During the dPCR reaction, the PICOglue Labels are amplified and fluorescent signal is generated and recorded. After the dPCR run, the raw data is uploaded and analyzed with our online software. The AMC-Kit contains materials to perform 120 dPCR reactions in 24-well dPCR plates or 480 dPCR reactions in 96-well dPCR plates. The following PICO products are required additionally for the workflow: - [PICO P8 Probe](https://pico-bioscience.myshopify.com/products/pico-p8-probe?utm_source=copyToPasteBoard&utm_medium=product-links&utm_content=web) - [PICO BL Probe](https://pico-bioscience.myshopify.com/products/pico-bl-probe) - [PICO N6 Probe](https://pico-bioscience.myshopify.com/products/pico-n6-probe) - [PICO O7 Probe](https://pico-bioscience.myshopify.com/products/pico-O7-probe) [Learn more](https://actome.myshopify.com/products/pico-amc-kit?variant=) ![PICO Antibody Labeling Service image](https://www.pico-bioscience.eu/hs-fs/hubfs/PICO_Labeling_Service-1.png?width=700&name=PICO_Labeling_Service-1.png) ## PICO Antibody Labeling Service 2.000,00 € We offer aprofessional service for labeling antibodies of your choice with PICOglue Labels, providing a highly sensitive and specific probe for various PICO applications.  Our service uses the **PICOglue Labeling Technology** to ensure the superior quality and reliability of the labeled antibodies. We guarantee at least 75% labeling efficiency (many times close to 100%), with a labeled antibody stability of at least 12 months. The yield is high and the amount of labeled antibodies are enough for thousands of PICO assays. The expected concentration of the labeled antibody is in the range of 5 × e10 - 5 × e12 cp/µL (copies per microliter). **Contact us** below for a quote! [Contact us](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) ![PICO EV Q Kit image](https://cdn.shopify.com/s/files/1/0042/1026/2051/files/Products-22-EVQ_Kit_Tubes.png?v=1781705330) ## PICO EV Q Kit 1.500,00 € The **PICO EV Q Kit** enables the absolute quantification and characterization of Extracellular vesicles based on their surface markers.  The workflow includes the incubation of your purified EVs with labeled antibodies. After incubation, the sample is diluted and loaded onto a dPCR plate. During the dPCR reaction, the PICOglue Labels are amplified, generating a fluorescent signal that is recorded. After the dPCR run, the raw data is uploaded and analyzed with our online software. The PICO EV Q Kit contains materials to perform 150 dPCR reactions in 24-well dPCR plates or 600 dPCR reactions in 96-well dPCR plates. The following PICO products are required additionally for the workflow: - [PICO P8 Probe](https://pico-bioscience.myshopify.com/products/pico-p8-probe?utm_source=copyToPasteBoard&utm_medium=product-links&utm_content=web) - [PICO BL Probe](https://pico-bioscience.myshopify.com/products/pico-bl-probe) - [PICO N6 Probe](https://pico-bioscience.myshopify.com/products/pico-n6-probe) - [PICO O7 Probe](https://pico-bioscience.myshopify.com/products/pico-O7-probe) [Contact us](https://www.pico-bioscience.eu/en/contact-us?hsLang=eu) ### **Trusted by Innovators in Life Sciences** ![Logo-Albert-Ludwigs-Universität\_Freiburg](https://www.pico-bioscience.eu/hubfs/Logo-Albert-Ludwigs-Universit%C3%A4t_Freiburg.svg) ![Imtek Logo](https://www.pico-bioscience.eu/hs-fs/hubfs/Imtek%20Logoi.jpeg?width=400&height=293&name=Imtek%20Logoi.jpeg) ![INTOEV\_logo](https://www.pico-bioscience.eu/hs-fs/hubfs/Logos/INTOEV_logo.png?width=211&height=113&name=INTOEV_logo.png) ## Upcoming Events ![FSEV-2026](https://25306495.fs1.hubspotusercontent-eu1.net/hub/25306495/hubfs/FSEV-2026.png?width=1200&length=1200&name=FSEV-2026.png) ### **Meet Us at FSEV 2026 in Paris!** Discover unmatched bench-to-bench reproducibility (CV \< 10%) for size-independent EV quantification with PICO BioScience at the 9th Meeting of the French Society of Extracellular Vesicles. - **When:** October 6th–8th, 2026 - **Where:** Paris, France - **Location:** Booth #21 Stop by our booth to explore our latest validation data and eliminate optical size bias from your workflow. **PICO makes it count!** [![GEIVEX 2026](https://25306495.fs1.hubspotusercontent-eu1.net/hub/25306495/hubfs/GEIVEX2026.jpg?width=1200&length=1200&name=GEIVEX2026.jpg)](https://www.geivex2026.com/) ### **Meet Us at GEIVEX 2026 in Bilbao!** Discover unmatched bench-to-bench reproducibility (CV \< 10%) for size-independent EV quantification with PICO BioScience at the meeting of the Spanish Innovation Network in Extracellular Vesicles. - **When:** October 6th–9th, 2026 - **Where:** Bilbao, Spain - **Location:** Co-exhibiting with HansaBioMed at Booth #12 Stop by our joint booth to explore our latest validation data and eliminate optical size bias from your workflow. **PICO makes it count!** [![health-innovation-nexus-Strasbourg](https://25306495.fs1.hubspotusercontent-eu1.net/hub/25306495/hubfs/health-innovation-nexus-Strasbourg.webp?width=1200&length=1200&name=health-innovation-nexus-Strasbourg.webp)](https://www.health-innovation-nexus.com/) ### **Meet Us in Strasbourg!** We are on the Stage at HEALTH INNOVATION NEXUS in Strasbourg. - **When:** November 6, 2026 - **Where:** Strasbourg, France - **Location:** IRCAD  1 place de l'Hôpital 67000 Strasbourg  France ![FSEV-2026](https://25306495.fs1.hubspotusercontent-eu1.net/hub/25306495/hubfs/FSEV-2026.png?width=1200&length=1200&name=FSEV-2026.png) ![GEIVEX 2026](https://25306495.fs1.hubspotusercontent-eu1.net/hub/25306495/hubfs/GEIVEX2026.jpg?width=1200&length=1200&name=GEIVEX2026.jpg) ![health-innovation-nexus-Strasbourg](https://25306495.fs1.hubspotusercontent-eu1.net/hub/25306495/hubfs/health-innovation-nexus-Strasbourg.webp?width=1200&length=1200&name=health-innovation-nexus-Strasbourg.webp) ## Management Team ![Tobias wo Background-1](https://www.pico-bioscience.eu/hs-fs/hubfs/Tobias%20wo%20Background-1.png?width=646&height=808&name=Tobias%20wo%20Background-1.png) ### Tobias Gross Chief Executive Officer   ![Maike wo Background](https://www.pico-bioscience.eu/hs-fs/hubfs/Maike%20wo%20Background.png?width=640&height=808&name=Maike%20wo%20Background.png) ### Maike Smits Head of Administration   ![Pablo wo Background flip](https://www.pico-bioscience.eu/hs-fs/hubfs/Pablo%20wo%20Background%20flip.png?width=639&height=803&name=Pablo%20wo%20Background%20flip.png) ### Pablo Sanchez Martin Head of Research & Development   ![Nina](https://www.pico-bioscience.eu/hs-fs/hubfs/Nina.png?width=646&height=808&name=Nina.png) ### Nina Gross Head of Production and Quality Management   ![Damaris](https://www.pico-bioscience.eu/hs-fs/hubfs/Damaris.png?width=307&height=386&name=Damaris.png) ### Damaris Jankowski Head of Finance and Coordinator for Publicly Funded Projects ![Marius\_White\_Background](https://www.pico-bioscience.eu/hs-fs/hubfs/Marius_White_Background.png?width=646&height=808&name=Marius_White_Background.png) ### Marius Pollet Head of Commercial Operations **[Privacy Policy ·](https://www.pico-bioscience.eu/privacy-policy?hsLang=eu) [General Terms and Conditions](https://www.pico-bioscience.eu/general-terms-and-conditions-pico-bioscience?hsLang=eu) · [Imprint/Impressum](https://www.pico-bioscience.eu/imprint?hsLang=eu)**© 2026 **·** All rights reserved